By L. Sokoloff (auth.), F. Gonzalez-Lima, Th. Finkenstädt, H. Scheich (eds.)
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Extra info for Advances in Metabolic Mapping Techniques for Brain Imaging of Behavioral and Learning Functions
Circular areas of uniform density represent calibrated 131I-labeled gelatin standards autoradiographed together with brain sections and used for quantitative densitometric analysis. A. Control study in blind-folded conscious cat; note visual cortex (Vis Cx), lateral (LG) and medial (MG) geniculate nuclei. B. Autoradiogram of section of brain of same cat at another level showing also superior colliculi (SCol). C. Autoradiogram of brain section comparable to section in panel A from another conscious cat with both eyes open and retinae stimulated by photo-flashes at 6 per second.
The total tissue 14C content, C~, minus a series of terms representing the 14C concentrations in non-protein compounds in tissue i at the kill time. , C~, C;, and C;p, respectively, in Fig. 6A and 6B); the 14C contents of these non-protein pools must be subtracted from C~ to obtain P~. By 60 minutes after the pulse, however, C~, C;, C;p, and any a-[ 1-14C]ketoisocaproic acid that might possibly remain in the tissue normally fall to low levels. There are no other 14C-labeled products that need to be subtracted because the only immediate product of [l-14C]leucine metabolism, a-[ 1-14C]ketoisocaproic acid, loses its label to 14C02 during its further metabolism.
There are no other 14C-labeled products that need to be subtracted because the only immediate product of [l-14C]leucine metabolism, a-[ 1-14C]ketoisocaproic acid, loses its label to 14C02 during its further metabolism. To assure, however, that even these low levels of non-protein 14C and any other water-soluble, non-protein labeled compounds that might be present are effectively removed, the frozen brain sections are fixed in 10% formalin prior to autoradiography. This procedure washes out the water-soluble labeled compounds but precipitates and retains the labeled protein.
Advances in Metabolic Mapping Techniques for Brain Imaging of Behavioral and Learning Functions by L. Sokoloff (auth.), F. Gonzalez-Lima, Th. Finkenstädt, H. Scheich (eds.)